Test Code RSV EIA/RSV DFA Respiratory Syncytial Virus by EIA or DFA
Performing Laboratory
CCHS-Christiana Care Health ServicesMethodology
Enzyme Immunoassay (EIA) or Direct Fluorescent Antigen (DFA)Reference Values
NegativePhysician Office Specimen Requirements
Specimen must be delivered to laboratory within 1 hour of collection.
Acceptable Specimens:
Bronchoalveolar lavage, bronchial washing, endoctracheal secretion, nasopharyngeal aspirate, nasopharyngeal swab, nasopharyngeal washing, throat swab, throat washing
Submit only 1 of the following specimens:
Preferred:
Nasopharyngeal Aspirate or Nasopharyngeal Washing
Container/Tube: Screw-capped, sterile container
Specimen: Nasopharyngeal aspirate or washing
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Nasopharyngeal Swab
Container/Tube: Culture transport swab
Specimen: Swab of nasopharyngeal
Collection Instructions: 1. Obtain specimen using flocked nasopharyngeal swab supplied.
2. Gently slide swab along floor of nose with very light pressure to nasopharyngeal wall.
3. Rotate swab slowly for 5 seconds to absorb secretions and remove along floor of nose.
4. Place swab in viral transport media.
5. Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Acceptable:
Bronchoalveolar Lavage or Bronchial Washing
Container/Tube: Screw-capped, sterile container
Specimen: 2 mL of bronchoalveolar lavage or bronchial washing
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Endotracheal Aspirate
Container/Tube: Screw-capped, sterile container
Specimen: 2 mL of endotracheal aspirate
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Throat Swab
Container/Tube: Dacron® swab supplied
Specimen: Swab of throat
Collection Instructions: 1. Tilt patient’s head back to assist in opening of mouth as wide as possible.
2. Depress tongue with a tongue depressor so swab does not touch oral mucosa or tongue.
3. If patient has complained of 1 spot being sore, swab that area well.
4. In 1 continuous motion:
A. Swab 1 tonsillar area up then down.
B. Move to back of throat as far down as possible and swab there.
C. Move to other tonsillar area and swab.
D. Swab behind uvula and remove swab.
5. Place swab in viral transport media.
6. Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Throat Washing
Container/Tube: Screw-capped, sterile container
Specimen: Throat washing
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Throat Swab
Container/Tube: Culture transport swab
Specimen: Swab of throat
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Day(s) Test Set Up
Monday through SundayRoutine Turnaround Time
DFA: 24 hours
EIA: 1 hour
STAT Turnaround Time
DFA: 24 hours
EIA: 1 hour