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Test Code MDIF Differential Leukocyte Count, Whole Blood

Performing Laboratory

ChristianaCare:

  • Newark Campus
  • Middletown Campus
  • Helen Graham Cancer Center
  • Wilmington Campus

Methodology

Manual—Microscopic Examination of Cells

Includes neutrophilic segs, neutrophilic bands, lymphocytes, monocytes, eosinophils, basophils, erythrocyte morphology, and platelets.

Reference Values

SEGMENTED NEUTROPHILS

Males

0-5 years:  not established

6-11 years:  29.0-66.0%

12-15 years:  28.0-78.0%

≥16 years:  42.0-75.0%

Females

0-5 years:  not established

6-11 years:  28.0-68.0%

12-15 years:  33.0-77.0%

≥16 years:  42.0-75.0%

BAND NEUTROPHILS

Males

0-5 years:  not established

6-11 years:  0.0-2.0%

≥12 years:  0.0-3.0%

Females

0-5 years:  not established

≥6 years:  0.0-3.0%

LYMPHOCYTES

Males

0-5 years:  not established

6-11 years:  23.0-64.0%

12-15 years:  18.0-54.0%

≥16 years:  16.0-52.0%

Females

0-5 years:  not established

6-11 years:  25.0-64.0%

12-15 years:  20.0-58.0%

≥16 years:  16.0-52.0%

MONOCYTES

Males

0-5 years:  3.0-10.0%

6-11 years:  1.0-13.0%

12-15 years:  1.0-12.0%

≥16 years:  1.0-11.0%

Females

0-5 years:  3.0-10.0%

6-11 years:  1.0-10.0%

≥12 years:  1.0-11.0%

EOSINOPHILS

Males

0-5 years:  0.0-8.0%

6-11 years:  0.0-11.0%

12-15 years:  0.0-9.0%

≥16 years:  0.0-7.0%

Females

0-5 years:  0.0-8.0%

6-11 years:  0.0-11.0%

12-15 years:  0.0-8.0%

≥16 years:  0.0-7.0%

BASOPHILS

Males

0-11 years:  0.0-3.0%

12-15 years:  0.0-2.0%

≥16 years:  0.0-4.0%

Females

0-15 years:  0.0-3.0%

≥16 years:  0.0-4.0%

METAMYELOCYTES

0-5 years:  not established

6-15 years:  0.0-0.0%

≥16 years:  <1.0%

MYELOCYTES

0-5 years:  not established

6-15 years:  0.0-0.0%

≥16 years:  <0.5%

Physician Office Specimen Requirements

Submit only 1 of the following specimens:

Blood

Specimen must arrive within 24 hours of draw.

 

Container/Tube:  3.5-mL lavender-top (EDTA) tube(s)

Specimen:  Full tube

Collection Instructions:  For pediatrics, draw 1.5 mL peripheral blood in a lavender-top (EDTA) MICROTAINER®.

Note:  Label tube(s) with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of draw, collector’s initials, and type of specimen.

 

Fingerstick Smears

Prepare fresh fingerstick smears (minimum volume:  1 well-made, unstained, peripheral blood smear from fingerstick). Forward promptly at ambient temperature only.

Note:  Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of draw, collector’s initials, and type of specimen.

Day(s) Test Set Up

Monday through Sunday

Routine Turnaround Time:  8 hours

STAT Turnaround Time:  1 hour