Test Code C CF Culture, Cystic Fibrosis, Respiratory
Performing Laboratory
CCHS-Christiana Care Health ServicesMethodology
CultureIncludes Gram stain.
Reference Values
No growth or oral floraPhysician Office Specimen Requirements
Specimen must be delivered to laboratory within 1 hour of collection.
Acceptable Specimens:
Bronchoalveolar lavage, endotracheal aspirate, lung biopsy, sputum, and throat swab
Submit only 1 of the following specimens:
Bronchoalveolar Lavage
Container/Tube: Screw-capped, sterile container
Specimen: Bronchoalveolar lavage
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Endotracheal Aspirate
Container/Tube: Screw-capped, sterile container
Specimen: Endotracheal aspirate
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Lung Biopsy
Container/Tube: Screw-capped, sterile container
Specimen: Biopsy of lung
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Sputum
Container/Tube: Screw-capped, sterile container
Specimen: First-morning sputum
Collection Instructions: Instruct patient to brush his/her teeth and/or rinse mouth well with water to minimize contaminating specimen with food particles, mouthwash, or oral drugs. Have patient remove dentures. Instruct patient to take a deep breath, hold it momentarily, then cough deeply and vigorously into screw-capped, sterile container. Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Throat Swab
Container/Tube: Culture transport tube
Specimen: Swab of throat
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Day(s) Test Set Up
Monday through SundayRoutine Turnaround Time
Gram stain: <8 hours
Preliminary: 24 hours
Final: 4 days
STAT Turnaround Time
Gram stain: ≤1 hour