Test Code C BF Culture, Body Fluid
Performing Laboratory
CCHS-Christiana Care Health ServicesMethodology
CultureIncludes Gram stain.
Reference Values
No growthPhysician Office Specimen Requirements
Specimen must be delivered to laboratory within 1 hour of collection.
Submit only 1 of the following specimens:
Ascites Fluid
Container/Tube: BACTECTM aerobic bottle (grey label), and a BACTECTM anaerobic bottle (purple label), and a SPS tube
Specimen: 23 mL of ascites fluid - 10 mL in each, BACTECTM aerobic bottle, and BACTECTM anaerobic bottle, and 3 mL in SPS tube for Gram stain.
Transport Temperature: Ambient - Do not place on wet ice or refrigerate.
Collection Instructions: Specimen should be collected by invasive radiology (direct image-guided aspiration). Maintain sterility and forward promptly at ambient temperature only.
Note: 1. Indicate ascites fluid on request form.
2. Label bottles with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Body Fluid
Container/Tube: Sterile, leakproof tube; aerobic yellow-top SPS tube, BBLTM Port-A-CulTM vial
Specimen: 1 mL to 5 mL (minimum volume: 1 mL) of body fluid
Transport Temperature: Ambient - Do not place on wet ice or refrigerate.
Collection Instructions: Specimen should be collected by percutaneous or intra-operative aspiration using aseptic technique. It is recommended that fluid be collected with a sterile needle and syringe, that any air bubbles present in the syringe be expelled, and body fluid be aseptically injected into an anaerobic fluid transport vial. Maintain sterility and forward promptly at ambient temperature only.
Note: 1. Indicate body fluid on request form.
2. Label tube/vial with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Day(s) Test Set Up
Monday through SundayRoutine Turnaround Time
Preliminary: 24 hours
Final: 3 to 5 days
STAT Turnaround Time: not available