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Test Code C AFB Culture, Mycobacterial/Acid Fast Bacillus (AFB), Body Fluid, Bronchial Washings, Spinal Fluid, Sputum, or Urine

Performing Laboratory

CCHS-Christiana Care Health Services

Methodology

Culture

Reference Values

Negative

Physician Office Specimen Requirements

Specimen must be delivered to laboratory within 1 hour of collection.

 

Acceptable Specimens:

Body fluid, bronchial washings, spinal fluid, sputum, tissue, and urine

 

Unacceptable Specimens:

Swabs are not recommended for isolation of mycobacteria and should not be used.

 

Submit only 1 of the following specimens:

 

Body Fluid

Container/Tube:  Screw-capped, sterile container

Specimen:  10 mL to 15 mL of body fluid

Collection Instructions:  Maintain sterility and forward promptly.

Note:  Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.

 

Bronchial Washings

Container/Tube:  Screw-capped, sterile container

Specimen:  5 mL to 10 mL of bronchial washings

Collection Instructions:  Maintain sterility and forward promptly.

Note:  Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.

 

Spinal Fluid

Container/Tube:  Screw-capped, sterile container

Specimen:  ≥2 mL of spinal fluid

Transport Temperature:  Ambient - Do not place on wet ice or refrigerate.

Collection Instructions:  Maintain sterility and forward promptly at ambient temperature only.

Note:  Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.

 

Sputum

Container/Tube:  Screw-capped, sterile container

Specimen:  5 mL to 10 mL of sputum

Collection Instructions:  Maintain sterility and forward promptly.

Note:  Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.

 

Tissue

Container/Tube:  Screw-capped, sterile container

Specimen:  Tissue - usually collected by physician

Collection Instructions:  Maintain sterility and forward promptly.

Note:  Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.

 

Urine

Container/Tube:  Screw-capped, sterile container

Specimen:  30 mL to 50 mL of urine

Collection Instructions:  Maintain sterility and forward promptly.

Note:  Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.

Day(s) Test Set Up

Monday through Sunday

Routine Turnaround Time

Stain:  24 hours from receipt in laboratory

Preliminary:  5 to 7 days

Final:  6 weeks

STAT Turnaround Time:  not available