Test Code C AFB Culture, Mycobacterial/Acid Fast Bacillus (AFB), Body Fluid, Bronchial Washings, Spinal Fluid, Sputum, or Urine
Performing Laboratory
CCHS-Christiana Care Health ServicesMethodology
CultureReference Values
NegativePhysician Office Specimen Requirements
Specimen must be delivered to laboratory within 1 hour of collection.
Acceptable Specimens:
Body fluid, bronchial washings, spinal fluid, sputum, tissue, and urine
Unacceptable Specimens:
Swabs are not recommended for isolation of mycobacteria and should not be used.
Submit only 1 of the following specimens:
Body Fluid
Container/Tube: Screw-capped, sterile container
Specimen: 10 mL to 15 mL of body fluid
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Bronchial Washings
Container/Tube: Screw-capped, sterile container
Specimen: 5 mL to 10 mL of bronchial washings
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Spinal Fluid
Container/Tube: Screw-capped, sterile container
Specimen: ≥2 mL of spinal fluid
Transport Temperature: Ambient - Do not place on wet ice or refrigerate.
Collection Instructions: Maintain sterility and forward promptly at ambient temperature only.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Sputum
Container/Tube: Screw-capped, sterile container
Specimen: 5 mL to 10 mL of sputum
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Tissue
Container/Tube: Screw-capped, sterile container
Specimen: Tissue - usually collected by physician
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Urine
Container/Tube: Screw-capped, sterile container
Specimen: 30 mL to 50 mL of urine
Collection Instructions: Maintain sterility and forward promptly.
Note: Label container with patient’s full name and 1 additional unique patient identifier such as date of birth, medical record number, emergency ID number, date and time of collection, collector’s initials, and type of specimen.
Day(s) Test Set Up
Monday through SundayRoutine Turnaround Time
Stain: 24 hours from receipt in laboratory
Preliminary: 5 to 7 days
Final: 6 weeks
STAT Turnaround Time: not available